Linked Production of Pyroglutamate-Modified Proteins via Self-Cleavage of Fusion Tags with TEV Protease and Autonomous N-Terminal Cyclization with Glutaminyl Cyclase In Vivo

Overproduction of N-terminal pyroglutamate (pGlu)-modified proteins utilizing Escherichia coli or eukaryotic cells is a challenging work owing to the fact that the recombinant proteins need to be recovered by proteolytic removal of fusion tags to expose the N-terminal glutaminyl or glutamyl residue,...

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Detalles Bibliográficos
Autores principales: Shih, Yan-Ping, Chou, Chi-Chi, Chen, Yi-Ling, Huang, Kai-Fa, Wang, Andrew H.- J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986218/
https://www.ncbi.nlm.nih.gov/pubmed/24733552
http://dx.doi.org/10.1371/journal.pone.0094812

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3986218/
https://www.ncbi.nlm.nih.gov/pubmed/24733552
http://dx.doi.org/10.1371/journal.pone.0094812

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