Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases

Flow cytometric analysis of p38 mitogen-activated protein kinase (p38 MAPK) signaling cascade is optimally achieved by methanol permeabilization protocols. Such protocols suffer from the difficulties to accurately detect intracellular cytokines and surface epitopes of infrequent cell subpopulations,...

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Detalles Bibliográficos
Autores principales: Mavropoulos, Athanasios, Bogdanos, Dimitrios P., Liaskos, Christos, Orfanidou, Timoklia, Simopoulou, Theodora, Zafiriou, Efterpi, Sakkas, Lazaros I., Rigopoulou, Eirini I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3987974/
https://www.ncbi.nlm.nih.gov/pubmed/24741615
http://dx.doi.org/10.1155/2014/671431

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3987974/
https://www.ncbi.nlm.nih.gov/pubmed/24741615
http://dx.doi.org/10.1155/2014/671431

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