A Mutant of Uracil DNA Glycosylase That Distinguishes between Cytosine and 5-Methylcytosine

We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme’s active site. Uracil is selected over cytosine by a pat...

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Detalles Bibliográficos
Autores principales: Kimber, Scott T., Brown, Tom, Fox, Keith R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989344/
https://www.ncbi.nlm.nih.gov/pubmed/24740413
http://dx.doi.org/10.1371/journal.pone.0095394

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989344/
https://www.ncbi.nlm.nih.gov/pubmed/24740413
http://dx.doi.org/10.1371/journal.pone.0095394

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