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Immobilised Histidine Tagged β (2)-Adrenoceptor Oriented by a Diazonium Salt Reaction and Its Application in Exploring Drug-Protein Interaction Using Ephedrine and Pseudoephedrine as Probes
A new oriented method using a diazonium salt reaction was developed for linking β (2)-adrenoceptor (β (2)-AR) on the surface of macroporous silica gel. Stationary phase containing the immobilised receptor was used to investigate the interaction between β (2)-AR and ephedrine plus pseudoephedrine by...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991622/ https://www.ncbi.nlm.nih.gov/pubmed/24747442 http://dx.doi.org/10.1371/journal.pone.0094955 |