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A Simple and Effective Method for Construction of Escherichia coli Strains Proficient for Genome Engineering
Multiplex genome engineering is a standalone recombineering tool for large-scale programming and accelerated evolution of cells. However, this advanced genome engineering technique has been limited to use in selected bacterial strains. We developed a simple and effective strain-independent method fo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991648/ https://www.ncbi.nlm.nih.gov/pubmed/24747264 http://dx.doi.org/10.1371/journal.pone.0094266 |