A Confirmation of the Quench-Cryoannealing Relaxation Protocol for Identifying Reduction States of Freeze-Trapped Nitrogenase Intermediates

[Image: see text] We have advanced a mechanism for nitrogenase catalysis that rests on the identification of a low-spin EPR signal (S = 1/2) trapped during turnover of a MoFe protein as the E(4) state, which has accumulated four reducing equivalents as two [Fe–H–Fe] bridging hydrides. Because electr...

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Detalles Bibliográficos
Autores principales: Lukoyanov, Dmitriy, Yang, Zhi-Yong, Duval, Simon, Danyal, Karamatullah, Dean, Dennis R., Seefeldt, Lance C., Hoffman, Brian M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993915/
https://www.ncbi.nlm.nih.gov/pubmed/24635454
http://dx.doi.org/10.1021/ic500013c

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993915/
https://www.ncbi.nlm.nih.gov/pubmed/24635454
http://dx.doi.org/10.1021/ic500013c

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