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A Confirmation of the Quench-Cryoannealing Relaxation Protocol for Identifying Reduction States of Freeze-Trapped Nitrogenase Intermediates
[Image: see text] We have advanced a mechanism for nitrogenase catalysis that rests on the identification of a low-spin EPR signal (S = 1/2) trapped during turnover of a MoFe protein as the E(4) state, which has accumulated four reducing equivalents as two [Fe–H–Fe] bridging hydrides. Because electr...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American
Chemical Society
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993915/ https://www.ncbi.nlm.nih.gov/pubmed/24635454 http://dx.doi.org/10.1021/ic500013c |