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A detailed analysis of next generation sequencing reads of microRNA expression in Barrett’s Esophagus: absolute versus relative quantification

BACKGROUND: Next generation sequencing (NGS) is a state of the art technology for microRNA (miRNA) analysis. The quantitative interpretation of the primary output of NGS i.e. the read counts for a miRNA sequence that can vary by several orders of magnitude (1 to 10(7)) remains incompletely understoo...

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Detalles Bibliográficos
Autores principales: Lee, In-Hee, Hong, Xiaoman, Mathur, Sharad C, Sharma, Mukut, Rastogi, Amit, Sharma, Prateek, Christenson, Lane K, Bansal, Ajay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994199/
https://www.ncbi.nlm.nih.gov/pubmed/24708854
http://dx.doi.org/10.1186/1756-0500-7-212