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Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis

BACKGROUND: There is conflicting evidence about the importance of airway mucins (MUC5AC and MUC5B) in determining physical properties of sputum in cystic fibrosis (CF). We studied the effects of endogenous degradation of mucins on CF sputum elasticity and apparent mucin concentrations. METHODS: Elas...

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Autores principales: Horsley, Alex, Rousseau, Karine, Ridley, Caroline, Flight, William, Jones, Andrew, Waigh, Thomas A., Thornton, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994278/
https://www.ncbi.nlm.nih.gov/pubmed/24332705
http://dx.doi.org/10.1016/j.jcf.2013.11.002
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author Horsley, Alex
Rousseau, Karine
Ridley, Caroline
Flight, William
Jones, Andrew
Waigh, Thomas A.
Thornton, David J.
author_facet Horsley, Alex
Rousseau, Karine
Ridley, Caroline
Flight, William
Jones, Andrew
Waigh, Thomas A.
Thornton, David J.
author_sort Horsley, Alex
collection PubMed
description BACKGROUND: There is conflicting evidence about the importance of airway mucins (MUC5AC and MUC5B) in determining physical properties of sputum in cystic fibrosis (CF). We studied the effects of endogenous degradation of mucins on CF sputum elasticity and apparent mucin concentrations. METHODS: Elastic shear moduli (G′) and mucin concentrations in sputum of 12 CF patients were measured before and after incubation at 37 °C for 60 min. RESULTS: G′ fell from a median of 5.98 to 4.70 Pa (p = 0.01). There were significant falls in MUC5AC (8.2 to 5.2 μg/ml, p = 0.02) and MUC5B (17.3 to 12.5 μg/ml, p = 0.02) over the same period, and associated decrease in molecular weight and size. CONCLUSIONS: Sputum is not inert and degradation reduces apparent mucin concentrations and sputum elasticity. Even if care is taken to process samples rapidly, sputum may therefore differ from secretions retained in airways. Previous studies may have underestimated the role of mucins in CF sputum.
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spelling pubmed-39942782014-05-01 Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis Horsley, Alex Rousseau, Karine Ridley, Caroline Flight, William Jones, Andrew Waigh, Thomas A. Thornton, David J. J Cyst Fibros Original Article BACKGROUND: There is conflicting evidence about the importance of airway mucins (MUC5AC and MUC5B) in determining physical properties of sputum in cystic fibrosis (CF). We studied the effects of endogenous degradation of mucins on CF sputum elasticity and apparent mucin concentrations. METHODS: Elastic shear moduli (G′) and mucin concentrations in sputum of 12 CF patients were measured before and after incubation at 37 °C for 60 min. RESULTS: G′ fell from a median of 5.98 to 4.70 Pa (p = 0.01). There were significant falls in MUC5AC (8.2 to 5.2 μg/ml, p = 0.02) and MUC5B (17.3 to 12.5 μg/ml, p = 0.02) over the same period, and associated decrease in molecular weight and size. CONCLUSIONS: Sputum is not inert and degradation reduces apparent mucin concentrations and sputum elasticity. Even if care is taken to process samples rapidly, sputum may therefore differ from secretions retained in airways. Previous studies may have underestimated the role of mucins in CF sputum. Elsevier 2014-05 /pmc/articles/PMC3994278/ /pubmed/24332705 http://dx.doi.org/10.1016/j.jcf.2013.11.002 Text en © 2013 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved. https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Original Article
Horsley, Alex
Rousseau, Karine
Ridley, Caroline
Flight, William
Jones, Andrew
Waigh, Thomas A.
Thornton, David J.
Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis
title Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis
title_full Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis
title_fullStr Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis
title_full_unstemmed Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis
title_short Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis
title_sort reassessment of the importance of mucins in determining sputum properties in cystic fibrosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994278/
https://www.ncbi.nlm.nih.gov/pubmed/24332705
http://dx.doi.org/10.1016/j.jcf.2013.11.002
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