Site-specific functionalization of proteins and their applications to therapeutic antibodies

Protein modifications are often required to study structure and function relationships. Instead of the random labeling of lysine residues, methods have been developed to (sequence) specific label proteins. Next to chemical modifications, tools to integrate new chemical groups for bioorthogonal react...

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Detalles Bibliográficos
Autores principales: van Vught, Remko, Pieters, Roland J, Breukink, Eefjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology (RNCSB) Organization 2014
Materias:
Mini Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995230/
https://www.ncbi.nlm.nih.gov/pubmed/24757499
http://dx.doi.org/10.5936/csbj.201402001
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author van Vught, Remko
Pieters, Roland J
Breukink, Eefjan
author_facet van Vught, Remko
Pieters, Roland J
Breukink, Eefjan
author_sort van Vught, Remko
collection PubMed
description Protein modifications are often required to study structure and function relationships. Instead of the random labeling of lysine residues, methods have been developed to (sequence) specific label proteins. Next to chemical modifications, tools to integrate new chemical groups for bioorthogonal reactions have been applied. Alternatively, proteins can also be selectively modified by enzymes. Herein we review the methods available for site-specific modification of proteins and their applications for therapeutic antibodies.
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spelling pubmed-39952302014-04-22 Site-specific functionalization of proteins and their applications to therapeutic antibodies van Vught, Remko Pieters, Roland J Breukink, Eefjan Comput Struct Biotechnol J Mini Review Protein modifications are often required to study structure and function relationships. Instead of the random labeling of lysine residues, methods have been developed to (sequence) specific label proteins. Next to chemical modifications, tools to integrate new chemical groups for bioorthogonal reactions have been applied. Alternatively, proteins can also be selectively modified by enzymes. Herein we review the methods available for site-specific modification of proteins and their applications for therapeutic antibodies. Research Network of Computational and Structural Biotechnology (RNCSB) Organization 2014-02-14 /pmc/articles/PMC3995230/ /pubmed/24757499 http://dx.doi.org/10.5936/csbj.201402001 Text en © van Vught et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly cited.
spellingShingle Mini Review
van Vught, Remko
Pieters, Roland J
Breukink, Eefjan
Site-specific functionalization of proteins and their applications to therapeutic antibodies
title Site-specific functionalization of proteins and their applications to therapeutic antibodies
title_full Site-specific functionalization of proteins and their applications to therapeutic antibodies
title_fullStr Site-specific functionalization of proteins and their applications to therapeutic antibodies
title_full_unstemmed Site-specific functionalization of proteins and their applications to therapeutic antibodies
title_short Site-specific functionalization of proteins and their applications to therapeutic antibodies
title_sort site-specific functionalization of proteins and their applications to therapeutic antibodies
topic Mini Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995230/
https://www.ncbi.nlm.nih.gov/pubmed/24757499
http://dx.doi.org/10.5936/csbj.201402001
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