Electroporation and Microinjection Successfully Deliver Single-Stranded and Duplex DNA into Live Cells as Detected by FRET Measurements

Förster resonance energy transfer (FRET) technology relies on the close proximity of two compatible fluorophores for energy transfer. Tagged (Cy3 and Cy5) complementary DNA strands forming a stable duplex and a doubly-tagged single strand were shown to demonstrate FRET outside of a cellular environm...

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Detalles Bibliográficos
Autores principales: Bamford, Rosemary A., Zhao, Zheng-yun, Hotchin, Neil A., Styles, Iain B., Nash, Gerard B., Tucker, James H. R., Bicknell, Roy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995676/
https://www.ncbi.nlm.nih.gov/pubmed/24755680
http://dx.doi.org/10.1371/journal.pone.0095097

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995676/
https://www.ncbi.nlm.nih.gov/pubmed/24755680
http://dx.doi.org/10.1371/journal.pone.0095097

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