Cargando…
Electroporation and Microinjection Successfully Deliver Single-Stranded and Duplex DNA into Live Cells as Detected by FRET Measurements
Förster resonance energy transfer (FRET) technology relies on the close proximity of two compatible fluorophores for energy transfer. Tagged (Cy3 and Cy5) complementary DNA strands forming a stable duplex and a doubly-tagged single strand were shown to demonstrate FRET outside of a cellular environm...
Autores principales: | Bamford, Rosemary A., Zhao, Zheng-yun, Hotchin, Neil A., Styles, Iain B., Nash, Gerard B., Tucker, James H. R., Bicknell, Roy |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995676/ https://www.ncbi.nlm.nih.gov/pubmed/24755680 http://dx.doi.org/10.1371/journal.pone.0095097 |
Ejemplares similares
-
Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
por: Kari, Willi, et al.
Publicado: (2016) -
Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection
por: Tazin, Nusrat, et al.
Publicado: (2023) -
Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection
por: Namula, Zhao, et al.
Publicado: (2022) -
Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods
por: Le, Quynh Anh, et al.
Publicado: (2022) -
Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
por: Le, Quynh Anh, et al.
Publicado: (2021)