Calcium Binding to Beta-2-Microglobulin at Physiological Ph Drives the Occurrence of Conformational Changes Which Cause the Protein to Precipitate into Amorphous Forms That Subsequently Transform into Amyloid Aggregates

Using spectroscopic, calorimetric and microscopic methods, we demonstrate that calcium binds to beta-2-microglobulin (β2m) under physiological conditions of pH and ionic strength, in biological buffers, causing a conformational change associated with the binding of up to four calcium atoms per β2m m...

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Detalles Bibliográficos
Autores principales: Kumar, Sukhdeep, Sharma, Prerna, Arora, Kanika, Raje, Manoj, Guptasarma, Purnananda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995793/
https://www.ncbi.nlm.nih.gov/pubmed/24755626
http://dx.doi.org/10.1371/journal.pone.0095725

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995793/
https://www.ncbi.nlm.nih.gov/pubmed/24755626
http://dx.doi.org/10.1371/journal.pone.0095725

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