A high-throughput, restriction-free cloning and screening strategy based on ccdB-gene replacement

BACKGROUND: In high-throughput demanding fields, such as biotechnology and structural biology, molecular cloning is an essential tool in obtaining high yields of recombinant protein. Here, we address recently developed restriction-free methods in cloning, and present a more cost-efficient protocol t...

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Detalles Bibliográficos
Autores principales: Lund, Bjarte Aarmo, Leiros, Hanna-Kirsti Schrøder, Bjerga, Gro Elin Kjæreng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3996003/
https://www.ncbi.nlm.nih.gov/pubmed/24612508
http://dx.doi.org/10.1186/1475-2859-13-38

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3996003/
https://www.ncbi.nlm.nih.gov/pubmed/24612508
http://dx.doi.org/10.1186/1475-2859-13-38

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