Cargando…
A high-throughput, restriction-free cloning and screening strategy based on ccdB-gene replacement
BACKGROUND: In high-throughput demanding fields, such as biotechnology and structural biology, molecular cloning is an essential tool in obtaining high yields of recombinant protein. Here, we address recently developed restriction-free methods in cloning, and present a more cost-efficient protocol t...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2014
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3996003/ https://www.ncbi.nlm.nih.gov/pubmed/24612508 http://dx.doi.org/10.1186/1475-2859-13-38 |