Molten Globule-Like Partially Folded State of Bacillus licheniformis α-Amylase at Low pH Induced by 1,1,1,3,3,3-Hexafluoroisopropanol

Effect of 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) on acid-denatured Bacillus licheniformis α-amylase (BLA) at pH 2.0 was investigated by far-UV CD, intrinsic fluorescence, and ANS fluorescence measurements. Addition of increasing HFIP concentrations led to an increase in the mean residue ellipticit...

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Detalles Bibliográficos
Autores principales: Abd Halim, Adyani Azizah, Zaroog, Mohammed Suleiman, Abdul Kadir, Habsah, Tayyab, Saad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3996958/
https://www.ncbi.nlm.nih.gov/pubmed/24977228
http://dx.doi.org/10.1155/2014/824768

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3996958/
https://www.ncbi.nlm.nih.gov/pubmed/24977228
http://dx.doi.org/10.1155/2014/824768

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