A simple method for the determination of reduction potentials in heme proteins

We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (E(m) = −115 mV ± 2 mV, pH 7.0) and human CLOCK (E(m) = −111 mV ± 2 mV, pH 7.0). We sugges...

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Autores principales: Efimov, Igor, Parkin, Gary, Millett, Elizabeth S., Glenday, Jennifer, Chan, Cheuk K., Weedon, Holly, Randhawa, Harpreet, Basran, Jaswir, Raven, Emma L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999514/
https://www.ncbi.nlm.nih.gov/pubmed/24440354
http://dx.doi.org/10.1016/j.febslet.2013.12.030
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author Efimov, Igor
Parkin, Gary
Millett, Elizabeth S.
Glenday, Jennifer
Chan, Cheuk K.
Weedon, Holly
Randhawa, Harpreet
Basran, Jaswir
Raven, Emma L.
author_facet Efimov, Igor
Parkin, Gary
Millett, Elizabeth S.
Glenday, Jennifer
Chan, Cheuk K.
Weedon, Holly
Randhawa, Harpreet
Basran, Jaswir
Raven, Emma L.
author_sort Efimov, Igor
collection PubMed
description We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (E(m) = −115 mV ± 2 mV, pH 7.0) and human CLOCK (E(m) = −111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.
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spelling pubmed-39995142014-09-03 A simple method for the determination of reduction potentials in heme proteins Efimov, Igor Parkin, Gary Millett, Elizabeth S. Glenday, Jennifer Chan, Cheuk K. Weedon, Holly Randhawa, Harpreet Basran, Jaswir Raven, Emma L. FEBS Lett Article We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (E(m) = −115 mV ± 2 mV, pH 7.0) and human CLOCK (E(m) = −111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins. Elsevier Science B.V 2014-03-03 /pmc/articles/PMC3999514/ /pubmed/24440354 http://dx.doi.org/10.1016/j.febslet.2013.12.030 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Efimov, Igor
Parkin, Gary
Millett, Elizabeth S.
Glenday, Jennifer
Chan, Cheuk K.
Weedon, Holly
Randhawa, Harpreet
Basran, Jaswir
Raven, Emma L.
A simple method for the determination of reduction potentials in heme proteins
title A simple method for the determination of reduction potentials in heme proteins
title_full A simple method for the determination of reduction potentials in heme proteins
title_fullStr A simple method for the determination of reduction potentials in heme proteins
title_full_unstemmed A simple method for the determination of reduction potentials in heme proteins
title_short A simple method for the determination of reduction potentials in heme proteins
title_sort simple method for the determination of reduction potentials in heme proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999514/
https://www.ncbi.nlm.nih.gov/pubmed/24440354
http://dx.doi.org/10.1016/j.febslet.2013.12.030
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