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Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing

BACKGROUND AND OBJECTIVE: The advent of next-generation sequencing has significantly facilitated characterization of the oral microbiome. Despite great efforts in streamlining the processes of sequencing and data curation, upstream steps required for amplicon library generation could still influence...

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Detalles Bibliográficos
Autores principales: Abusleme, Loreto, Hong, Bo-Young, Dupuy, Amanda K., Strausbaugh, Linda D., Diaz, Patricia I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Co-Action Publishing 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4000428/
https://www.ncbi.nlm.nih.gov/pubmed/24778776
http://dx.doi.org/10.3402/jom.v6.23990