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Mutations of Cx43 that affect B cell spreading in response to BCR signaling
The gap junction (GJ) protein connexin 43 (Cx43) is both necessary and sufficient for B cell receptor (BCR)-mediated cell spreading. To address how Cx43 mediates this effect, we blocked its function genetically, by expressing mutants of Cx43, and pharmacologically, by using chemical inhibitors. Whil...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4001238/ https://www.ncbi.nlm.nih.gov/pubmed/24526714 http://dx.doi.org/10.1242/bio.20147328 |
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author | Falk, Letitia Dang-Lawson, May Vega, José Luis Pournia, Farnaz Choi, Kate Jang, Caren Naus, Christian C. Matsuuchi, Linda |
author_facet | Falk, Letitia Dang-Lawson, May Vega, José Luis Pournia, Farnaz Choi, Kate Jang, Caren Naus, Christian C. Matsuuchi, Linda |
author_sort | Falk, Letitia |
collection | PubMed |
description | The gap junction (GJ) protein connexin 43 (Cx43) is both necessary and sufficient for B cell receptor (BCR)-mediated cell spreading. To address how Cx43 mediates this effect, we blocked its function genetically, by expressing mutants of Cx43, and pharmacologically, by using chemical inhibitors. While various point mutations of Cx43 inhibited B cell spreading, treatment with channel blocking drugs did not, suggesting that this response was independent of channel function. The critical region of Cx43 appears to be the cytoplasmic carboxyl-terminal (CT) domain, which has previously been shown to be important for B cell spreading. Consistent with this, mutations of either tyrosine 247 or 265 found in the CT were sufficient to inhibit spreading. Thus Cx43 may influence B cell spreading by mechanisms requiring protein binding to, or modification of, these sites in the CT tail. |
format | Online Article Text |
id | pubmed-4001238 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Company of Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-40012382014-06-02 Mutations of Cx43 that affect B cell spreading in response to BCR signaling Falk, Letitia Dang-Lawson, May Vega, José Luis Pournia, Farnaz Choi, Kate Jang, Caren Naus, Christian C. Matsuuchi, Linda Biol Open Research Article The gap junction (GJ) protein connexin 43 (Cx43) is both necessary and sufficient for B cell receptor (BCR)-mediated cell spreading. To address how Cx43 mediates this effect, we blocked its function genetically, by expressing mutants of Cx43, and pharmacologically, by using chemical inhibitors. While various point mutations of Cx43 inhibited B cell spreading, treatment with channel blocking drugs did not, suggesting that this response was independent of channel function. The critical region of Cx43 appears to be the cytoplasmic carboxyl-terminal (CT) domain, which has previously been shown to be important for B cell spreading. Consistent with this, mutations of either tyrosine 247 or 265 found in the CT were sufficient to inhibit spreading. Thus Cx43 may influence B cell spreading by mechanisms requiring protein binding to, or modification of, these sites in the CT tail. The Company of Biologists 2014-02-13 /pmc/articles/PMC4001238/ /pubmed/24526714 http://dx.doi.org/10.1242/bio.20147328 Text en © 2014. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Falk, Letitia Dang-Lawson, May Vega, José Luis Pournia, Farnaz Choi, Kate Jang, Caren Naus, Christian C. Matsuuchi, Linda Mutations of Cx43 that affect B cell spreading in response to BCR signaling |
title | Mutations of Cx43 that affect B cell spreading in response to BCR signaling |
title_full | Mutations of Cx43 that affect B cell spreading in response to BCR signaling |
title_fullStr | Mutations of Cx43 that affect B cell spreading in response to BCR signaling |
title_full_unstemmed | Mutations of Cx43 that affect B cell spreading in response to BCR signaling |
title_short | Mutations of Cx43 that affect B cell spreading in response to BCR signaling |
title_sort | mutations of cx43 that affect b cell spreading in response to bcr signaling |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4001238/ https://www.ncbi.nlm.nih.gov/pubmed/24526714 http://dx.doi.org/10.1242/bio.20147328 |
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