A high-throughput screening system for G-protein-coupled receptors using β-lactamase enzyme complementation technology

AIM: To establish a system for monitoring the activation of G-protein-coupled receptors (GPCRs) using β-lactamase enzyme fragment complementation (EFC) technology. METHODS: Two inactive β-lactamase deletion fragments, bla(a) and bla(b), were fused to β-arrestin and GPCR, respectively. A stable cell...

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Detalles Bibliográficos
Autores principales: Zhao, Chuan-ke, Yin, Qi, Li, Shi-you
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2010
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4002942/
https://www.ncbi.nlm.nih.gov/pubmed/21102483
http://dx.doi.org/10.1038/aps.2010.154

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4002942/
https://www.ncbi.nlm.nih.gov/pubmed/21102483
http://dx.doi.org/10.1038/aps.2010.154

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