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Low copy target detection by Droplet Digital PCR through application of a novel open access bioinformatic pipeline, ‘definetherain’

Droplet Digital PCR (ddPCR) represents a new and alternative platform to conventional quantitative-PCR (qPCR) for the quantitation of DNA templates. However, the proposed improvement in sensitivity and reproducibility offered by ddPCR is not yet fully proven, partly because the delineation between p...

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Detalles Bibliográficos
Autores principales: Jones, Mathew, Williams, James, Gärtner, Kathleen, Phillips, Rodney, Hurst, Jacob, Frater, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier/North-Holland Biomedical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4003534/
https://www.ncbi.nlm.nih.gov/pubmed/24598230
http://dx.doi.org/10.1016/j.jviromet.2014.02.020