Experimental and Computational Mutagenesis To Investigate the Positioning of a General Base within an Enzyme Active Site

[Image: see text] The positioning of catalytic groups within proteins plays an important role in enzyme catalysis, and here we investigate the positioning of the general base in the enzyme ketosteroid isomerase (KSI). The oxygen atoms of Asp38, the general base in KSI, were previously shown to be in...

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Detalles Bibliográficos
Autores principales: Schwans, Jason P., Hanoian, Philip, Lengerich, Benjamin J., Sunden, Fanny, Gonzalez, Ana, Tsai, Yingssu, Hammes-Schiffer, Sharon, Herschlag, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004248/
https://www.ncbi.nlm.nih.gov/pubmed/24597914
http://dx.doi.org/10.1021/bi401671t

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004248/
https://www.ncbi.nlm.nih.gov/pubmed/24597914
http://dx.doi.org/10.1021/bi401671t

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