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Ovariectomized rats as a model of postmenopausal osteoarthritis: validation and application

We aimed to assess the effect of ovariectomy on cartilage turnover and degradation, to evaluate whether ovariectomized (OVX) rats could form an experimental model of postmenopausal osteoarthritis. The effect of ovariectomy on cartilage was studied using two cohorts of female Sprague–Dawley rats, age...

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Autores principales: Høegh-Andersen, Pernille, Tankó, László B, Andersen, Thomas L, Lundberg, Carina V, Mo, John A, Heegaard, Anne-Marie, Delaissé, Jean-Marie, Christgau, Stephan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC400436/
https://www.ncbi.nlm.nih.gov/pubmed/15059281
http://dx.doi.org/10.1186/ar1152
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author Høegh-Andersen, Pernille
Tankó, László B
Andersen, Thomas L
Lundberg, Carina V
Mo, John A
Heegaard, Anne-Marie
Delaissé, Jean-Marie
Christgau, Stephan
author_facet Høegh-Andersen, Pernille
Tankó, László B
Andersen, Thomas L
Lundberg, Carina V
Mo, John A
Heegaard, Anne-Marie
Delaissé, Jean-Marie
Christgau, Stephan
author_sort Høegh-Andersen, Pernille
collection PubMed
description We aimed to assess the effect of ovariectomy on cartilage turnover and degradation, to evaluate whether ovariectomized (OVX) rats could form an experimental model of postmenopausal osteoarthritis. The effect of ovariectomy on cartilage was studied using two cohorts of female Sprague–Dawley rats, aged 5 and 7 months. In a third cohort, the effect of exogenous estrogen and a selective estrogen receptor modulator was analyzed. Knee joints were assessed by histological analysis of the articular cartilage after 9 weeks. Cartilage turnover was measured in urine by an immunoassay specific for collagen type II degradation products (CTX-II), and bone resorption was quantified in serum using an assay for bone collagen type I fragments (CTX-I). Surface erosion in the cartilage of the knee was more severe in OVX rats than in sham-operated animals, particularly in the 7-month-old cohort (P = 0.008). Ovariectomy also significant increased CTX-I and CTX-II. Both the absolute levels of CTX-II and the relative changes from baseline seen at week 4 correlated strongly with the severity of cartilage surface erosion at termination (r = 0.74, P < 0.01). Both estrogen and the selective estrogen receptor modulator inhibited the ovariectomy-induced acceleration of cartilage and bone turnover and significantly suppressed cartilage degradation and erosion seen in vehicle-treated OVX rats. The study indicates that estrogen deficiency accelerates cartilage turnover and increases cartilage surface erosion. OVX rats provide a useful experimental model for the evaluation of the chondroprotective effects of estrogens and estrogen-like substances and the model may be an in vivo representation of osteoarthritis in postmenopausal women.
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spelling pubmed-4004362004-04-30 Ovariectomized rats as a model of postmenopausal osteoarthritis: validation and application Høegh-Andersen, Pernille Tankó, László B Andersen, Thomas L Lundberg, Carina V Mo, John A Heegaard, Anne-Marie Delaissé, Jean-Marie Christgau, Stephan Arthritis Res Ther Research Article We aimed to assess the effect of ovariectomy on cartilage turnover and degradation, to evaluate whether ovariectomized (OVX) rats could form an experimental model of postmenopausal osteoarthritis. The effect of ovariectomy on cartilage was studied using two cohorts of female Sprague–Dawley rats, aged 5 and 7 months. In a third cohort, the effect of exogenous estrogen and a selective estrogen receptor modulator was analyzed. Knee joints were assessed by histological analysis of the articular cartilage after 9 weeks. Cartilage turnover was measured in urine by an immunoassay specific for collagen type II degradation products (CTX-II), and bone resorption was quantified in serum using an assay for bone collagen type I fragments (CTX-I). Surface erosion in the cartilage of the knee was more severe in OVX rats than in sham-operated animals, particularly in the 7-month-old cohort (P = 0.008). Ovariectomy also significant increased CTX-I and CTX-II. Both the absolute levels of CTX-II and the relative changes from baseline seen at week 4 correlated strongly with the severity of cartilage surface erosion at termination (r = 0.74, P < 0.01). Both estrogen and the selective estrogen receptor modulator inhibited the ovariectomy-induced acceleration of cartilage and bone turnover and significantly suppressed cartilage degradation and erosion seen in vehicle-treated OVX rats. The study indicates that estrogen deficiency accelerates cartilage turnover and increases cartilage surface erosion. OVX rats provide a useful experimental model for the evaluation of the chondroprotective effects of estrogens and estrogen-like substances and the model may be an in vivo representation of osteoarthritis in postmenopausal women. BioMed Central 2004 2004-02-19 /pmc/articles/PMC400436/ /pubmed/15059281 http://dx.doi.org/10.1186/ar1152 Text en Copyright © 2004 Høegh-Andersen et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Høegh-Andersen, Pernille
Tankó, László B
Andersen, Thomas L
Lundberg, Carina V
Mo, John A
Heegaard, Anne-Marie
Delaissé, Jean-Marie
Christgau, Stephan
Ovariectomized rats as a model of postmenopausal osteoarthritis: validation and application
title Ovariectomized rats as a model of postmenopausal osteoarthritis: validation and application
title_full Ovariectomized rats as a model of postmenopausal osteoarthritis: validation and application
title_fullStr Ovariectomized rats as a model of postmenopausal osteoarthritis: validation and application
title_full_unstemmed Ovariectomized rats as a model of postmenopausal osteoarthritis: validation and application
title_short Ovariectomized rats as a model of postmenopausal osteoarthritis: validation and application
title_sort ovariectomized rats as a model of postmenopausal osteoarthritis: validation and application
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC400436/
https://www.ncbi.nlm.nih.gov/pubmed/15059281
http://dx.doi.org/10.1186/ar1152
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