Cargando…
Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity
The nuclear pore complex (NPC) has dual roles in nucleocytoplasmic transport and chromatin organization. In many eukaryotes the coiled-coil Mlp/Tpr proteins of the NPC nuclear basket have specific functions in interactions with chromatin and defining specialized regions of active transcription, wher...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004592/ https://www.ncbi.nlm.nih.gov/pubmed/24600046 http://dx.doi.org/10.1091/mbc.E13-12-0750 |
_version_ | 1782313997364625408 |
---|---|
author | Holden, Jennifer M. Koreny, Ludek Obado, Samson Ratushny, Alexander V. Chen, Wei-Ming Chiang, Jung-Hsien Kelly, Steven Chait, Brian T. Aitchison, John D. Rout, Michael P. Field, Mark C. |
author_facet | Holden, Jennifer M. Koreny, Ludek Obado, Samson Ratushny, Alexander V. Chen, Wei-Ming Chiang, Jung-Hsien Kelly, Steven Chait, Brian T. Aitchison, John D. Rout, Michael P. Field, Mark C. |
author_sort | Holden, Jennifer M. |
collection | PubMed |
description | The nuclear pore complex (NPC) has dual roles in nucleocytoplasmic transport and chromatin organization. In many eukaryotes the coiled-coil Mlp/Tpr proteins of the NPC nuclear basket have specific functions in interactions with chromatin and defining specialized regions of active transcription, whereas Mlp2 associates with the mitotic spindle/NPC in a cell cycle–dependent manner. We previously identified two putative Mlp-related proteins in African trypanosomes, TbNup110 and TbNup92, the latter of which associates with the spindle. We now provide evidence for independent ancestry for TbNup92/TbNup110 and Mlp/Tpr proteins. However, TbNup92 is required for correct chromosome segregation, with knockout cells exhibiting microaneuploidy and lowered fidelity of telomere segregation. Further, TbNup92 is intimately associated with the mitotic spindle and spindle anchor site but apparently has minimal roles in control of gene transcription, indicating that TbNup92 lacks major barrier activity. TbNup92 therefore acts as a functional analogue of Mlp/Tpr proteins, and, together with the lamina analogue NUP-1, represents a cohort of novel proteins operating at the nuclear periphery of trypanosomes, uncovering complex evolutionary trajectories for the NPC and nuclear lamina. |
format | Online Article Text |
id | pubmed-4004592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-40045922014-07-16 Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity Holden, Jennifer M. Koreny, Ludek Obado, Samson Ratushny, Alexander V. Chen, Wei-Ming Chiang, Jung-Hsien Kelly, Steven Chait, Brian T. Aitchison, John D. Rout, Michael P. Field, Mark C. Mol Biol Cell Articles The nuclear pore complex (NPC) has dual roles in nucleocytoplasmic transport and chromatin organization. In many eukaryotes the coiled-coil Mlp/Tpr proteins of the NPC nuclear basket have specific functions in interactions with chromatin and defining specialized regions of active transcription, whereas Mlp2 associates with the mitotic spindle/NPC in a cell cycle–dependent manner. We previously identified two putative Mlp-related proteins in African trypanosomes, TbNup110 and TbNup92, the latter of which associates with the spindle. We now provide evidence for independent ancestry for TbNup92/TbNup110 and Mlp/Tpr proteins. However, TbNup92 is required for correct chromosome segregation, with knockout cells exhibiting microaneuploidy and lowered fidelity of telomere segregation. Further, TbNup92 is intimately associated with the mitotic spindle and spindle anchor site but apparently has minimal roles in control of gene transcription, indicating that TbNup92 lacks major barrier activity. TbNup92 therefore acts as a functional analogue of Mlp/Tpr proteins, and, together with the lamina analogue NUP-1, represents a cohort of novel proteins operating at the nuclear periphery of trypanosomes, uncovering complex evolutionary trajectories for the NPC and nuclear lamina. The American Society for Cell Biology 2014-05-01 /pmc/articles/PMC4004592/ /pubmed/24600046 http://dx.doi.org/10.1091/mbc.E13-12-0750 Text en © 2014 Holden et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Holden, Jennifer M. Koreny, Ludek Obado, Samson Ratushny, Alexander V. Chen, Wei-Ming Chiang, Jung-Hsien Kelly, Steven Chait, Brian T. Aitchison, John D. Rout, Michael P. Field, Mark C. Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity |
title | Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity |
title_full | Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity |
title_fullStr | Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity |
title_full_unstemmed | Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity |
title_short | Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity |
title_sort | nuclear pore complex evolution: a trypanosome mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004592/ https://www.ncbi.nlm.nih.gov/pubmed/24600046 http://dx.doi.org/10.1091/mbc.E13-12-0750 |
work_keys_str_mv | AT holdenjenniferm nuclearporecomplexevolutionatrypanosomemlpanaloguefunctionsinchromosomalsegregationbutlackstranscriptionalbarrieractivity AT korenyludek nuclearporecomplexevolutionatrypanosomemlpanaloguefunctionsinchromosomalsegregationbutlackstranscriptionalbarrieractivity AT obadosamson nuclearporecomplexevolutionatrypanosomemlpanaloguefunctionsinchromosomalsegregationbutlackstranscriptionalbarrieractivity AT ratushnyalexanderv nuclearporecomplexevolutionatrypanosomemlpanaloguefunctionsinchromosomalsegregationbutlackstranscriptionalbarrieractivity AT chenweiming nuclearporecomplexevolutionatrypanosomemlpanaloguefunctionsinchromosomalsegregationbutlackstranscriptionalbarrieractivity AT chiangjunghsien nuclearporecomplexevolutionatrypanosomemlpanaloguefunctionsinchromosomalsegregationbutlackstranscriptionalbarrieractivity AT kellysteven nuclearporecomplexevolutionatrypanosomemlpanaloguefunctionsinchromosomalsegregationbutlackstranscriptionalbarrieractivity AT chaitbriant nuclearporecomplexevolutionatrypanosomemlpanaloguefunctionsinchromosomalsegregationbutlackstranscriptionalbarrieractivity AT aitchisonjohnd nuclearporecomplexevolutionatrypanosomemlpanaloguefunctionsinchromosomalsegregationbutlackstranscriptionalbarrieractivity AT routmichaelp nuclearporecomplexevolutionatrypanosomemlpanaloguefunctionsinchromosomalsegregationbutlackstranscriptionalbarrieractivity AT fieldmarkc nuclearporecomplexevolutionatrypanosomemlpanaloguefunctionsinchromosomalsegregationbutlackstranscriptionalbarrieractivity |