The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs

The cyclic AMP sensor, EPAC1, activates AP1-mediated transcription in HUVECs. Correspondingly, induction of the SOCS3 minimal promoter by EPAC1 requires a single AP1 site that constitutively binds phosphorylated (Ser63) c-Jun in DNA-pull-down assays. c-Jun (Ser63) becomes further phosphorylated foll...

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Detalles Bibliográficos
Autores principales: Wiejak, Jolanta, Dunlop, Julia, Yarwood, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005914/
https://www.ncbi.nlm.nih.gov/pubmed/24631457
http://dx.doi.org/10.1016/j.febslet.2014.02.038
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author Wiejak, Jolanta
Dunlop, Julia
Yarwood, Stephen J.
author_facet Wiejak, Jolanta
Dunlop, Julia
Yarwood, Stephen J.
author_sort Wiejak, Jolanta
collection PubMed
description The cyclic AMP sensor, EPAC1, activates AP1-mediated transcription in HUVECs. Correspondingly, induction of the SOCS3 minimal promoter by EPAC1 requires a single AP1 site that constitutively binds phosphorylated (Ser63) c-Jun in DNA-pull-down assays. c-Jun (Ser63) becomes further phosphorylated following cyclic AMP stimulation and specific activation of protein kinase A (PKA), but not through selective activation of EPAC1. Moreover, despite a requirement for c-Jun for SOCS3 induction in fibroblasts, phospho-null c-Jun (Ser63/73Ala) had little effect on SOCS3 induction by cyclic AMP in HUVECs. AP1 activation and SOCS3 induction by EPAC1 in HUVECs therefore occur independently of c-Jun phosphorylation on Ser63.
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spelling pubmed-40059142014-05-02 The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs Wiejak, Jolanta Dunlop, Julia Yarwood, Stephen J. FEBS Lett Article The cyclic AMP sensor, EPAC1, activates AP1-mediated transcription in HUVECs. Correspondingly, induction of the SOCS3 minimal promoter by EPAC1 requires a single AP1 site that constitutively binds phosphorylated (Ser63) c-Jun in DNA-pull-down assays. c-Jun (Ser63) becomes further phosphorylated following cyclic AMP stimulation and specific activation of protein kinase A (PKA), but not through selective activation of EPAC1. Moreover, despite a requirement for c-Jun for SOCS3 induction in fibroblasts, phospho-null c-Jun (Ser63/73Ala) had little effect on SOCS3 induction by cyclic AMP in HUVECs. AP1 activation and SOCS3 induction by EPAC1 in HUVECs therefore occur independently of c-Jun phosphorylation on Ser63. Elsevier Science B.V 2014-05-02 /pmc/articles/PMC4005914/ /pubmed/24631457 http://dx.doi.org/10.1016/j.febslet.2014.02.038 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Wiejak, Jolanta
Dunlop, Julia
Yarwood, Stephen J.
The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs
title The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs
title_full The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs
title_fullStr The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs
title_full_unstemmed The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs
title_short The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs
title_sort role of c-jun in controlling the epac1-dependent induction of the socs3 gene in huvecs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005914/
https://www.ncbi.nlm.nih.gov/pubmed/24631457
http://dx.doi.org/10.1016/j.febslet.2014.02.038
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