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Resolving the titer of murine cytomegalovirus by plaque assay using the M2-10B4 cell line and a low viscosity overlay

BACKGROUND: Murine cytomegalovirus (MCMV) is increasingly used as an infectious model to investigate host-pathogen interactions in mice. Detailed methods have been published for using primary murine embryonic fibroblasts (MEFs) for preparing stocks and determining viral titers of MCMV. For determini...

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Detalles Bibliográficos
Autores principales: Zurbach, Katherine A, Moghbeli, Toktam, Snyder, Christopher M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4006460/
https://www.ncbi.nlm.nih.gov/pubmed/24742045
http://dx.doi.org/10.1186/1743-422X-11-71