Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA)

BACKGROUND: Segregational stability of plasmids is of major concern for recombinant bacterial production strains. One of the best strategies to counteract plasmid loss is the use of auxotrophic mutants which are complemented with the lacking gene along with the product-relevant ones. However, these...

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Detalles Bibliográficos
Autores principales: Velur Selvamani, Ram Shankar, Telaar, Maurice, Friehs, Karl, Flaschel, Erwin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4006690/
https://www.ncbi.nlm.nih.gov/pubmed/24745552
http://dx.doi.org/10.1186/1475-2859-13-58

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4006690/
https://www.ncbi.nlm.nih.gov/pubmed/24745552
http://dx.doi.org/10.1186/1475-2859-13-58

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