Expanded Genetic Codes in Next Generation Sequencing Enable Decontamination and Mitochondrial Enrichment

We have developed a PCR method, coined Déjà vu PCR, that utilizes six nucleotides in PCR with two methyl specific restriction enzymes that respectively digest these additional nucleotides. Use of this enzyme-and-nucleotide combination enables what we term a “DNA diode”, where DNA can advance in a la...

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Detalles Bibliográficos
Autores principales: McKernan, Kevin J., Spangler, Jessica, Zhang, Lei, Tadigotla, Vasisht, McLaughlin, Stephen, Warner, Jason, Zare, Amir, Boles, Richard G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4008621/
https://www.ncbi.nlm.nih.gov/pubmed/24788618
http://dx.doi.org/10.1371/journal.pone.0096492

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4008621/
https://www.ncbi.nlm.nih.gov/pubmed/24788618
http://dx.doi.org/10.1371/journal.pone.0096492

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