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Expanded Genetic Codes in Next Generation Sequencing Enable Decontamination and Mitochondrial Enrichment
We have developed a PCR method, coined Déjà vu PCR, that utilizes six nucleotides in PCR with two methyl specific restriction enzymes that respectively digest these additional nucleotides. Use of this enzyme-and-nucleotide combination enables what we term a “DNA diode”, where DNA can advance in a la...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4008621/ https://www.ncbi.nlm.nih.gov/pubmed/24788618 http://dx.doi.org/10.1371/journal.pone.0096492 |