In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors

Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active mole...

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Autores principales: Hoang, Thi-My-Nhung, Vu, Hong-Lien, Le, Ly-Thuy-Tram, Nguyen, Chi-Hung, Molla, Annie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009756/
https://www.ncbi.nlm.nih.gov/pubmed/24833340
http://dx.doi.org/10.3390/biology3010167
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author Hoang, Thi-My-Nhung
Vu, Hong-Lien
Le, Ly-Thuy-Tram
Nguyen, Chi-Hung
Molla, Annie
author_facet Hoang, Thi-My-Nhung
Vu, Hong-Lien
Le, Ly-Thuy-Tram
Nguyen, Chi-Hung
Molla, Annie
author_sort Hoang, Thi-My-Nhung
collection PubMed
description Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active molecules. We propose a decision tree that could be adapted to most in vitro HTS.
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spelling pubmed-40097562014-05-07 In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors Hoang, Thi-My-Nhung Vu, Hong-Lien Le, Ly-Thuy-Tram Nguyen, Chi-Hung Molla, Annie Biology (Basel) Article Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active molecules. We propose a decision tree that could be adapted to most in vitro HTS. MDPI 2014-02-27 /pmc/articles/PMC4009756/ /pubmed/24833340 http://dx.doi.org/10.3390/biology3010167 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Hoang, Thi-My-Nhung
Vu, Hong-Lien
Le, Ly-Thuy-Tram
Nguyen, Chi-Hung
Molla, Annie
In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors
title In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors
title_full In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors
title_fullStr In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors
title_full_unstemmed In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors
title_short In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors
title_sort in vitro high throughput screening, what next? lessons from the screening for aurora kinase inhibitors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009756/
https://www.ncbi.nlm.nih.gov/pubmed/24833340
http://dx.doi.org/10.3390/biology3010167
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