Targeted Toxin-Based Selectable Drug-Free Enrichment of Mammalian Cells with High Transgene Expression

Almost all transfection protocols for mammalian cells use a drug resistance gene for the selection of transfected cells. However, it always requires the characterization of each isolated clone regarding transgene expression, which is time-consuming and labor-intensive. In the current study, we devel...

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Detalles Bibliográficos
Autores principales: Sato, Masahiro, Akasaka, Eri, Saitoh, Issei, Ohtsuka, Masato, Nakamura, Shingo, Sakurai, Takayuki, Watanabe, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009860/
https://www.ncbi.nlm.nih.gov/pubmed/24832665
http://dx.doi.org/10.3390/biology2010341

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009860/
https://www.ncbi.nlm.nih.gov/pubmed/24832665
http://dx.doi.org/10.3390/biology2010341

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