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Targeted Toxin-Based Selectable Drug-Free Enrichment of Mammalian Cells with High Transgene Expression

Almost all transfection protocols for mammalian cells use a drug resistance gene for the selection of transfected cells. However, it always requires the characterization of each isolated clone regarding transgene expression, which is time-consuming and labor-intensive. In the current study, we devel...

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Detalles Bibliográficos
Autores principales:	Sato, Masahiro, Akasaka, Eri, Saitoh, Issei, Ohtsuka, Masato, Nakamura, Shingo, Sakurai, Takayuki, Watanabe, Satoshi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2013
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009860/ https://www.ncbi.nlm.nih.gov/pubmed/24832665 http://dx.doi.org/10.3390/biology2010341

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