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Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis

BACKGROUND: Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a...

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Detalles Bibliográficos
Autores principales: Gonçalves-de-Albuquerque, Suênia da Cunha, Pessoa e Silva, Rômulo, de Morais, Rayana Carla Silva, Trajano-Silva, Lays Adrianne Mendonça, Régis-da-Silva, Carlos Gustavo, Brandão-Filho, Sinval Pinto, de Paiva-Cavalcanti, Milena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012836/
https://www.ncbi.nlm.nih.gov/pubmed/24808911
http://dx.doi.org/10.1186/1678-9199-20-16