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Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
BACKGROUND: Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a...
Autores principales: | Gonçalves-de-Albuquerque, Suênia da Cunha, Pessoa e Silva, Rômulo, de Morais, Rayana Carla Silva, Trajano-Silva, Lays Adrianne Mendonça, Régis-da-Silva, Carlos Gustavo, Brandão-Filho, Sinval Pinto, de Paiva-Cavalcanti, Milena |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012836/ https://www.ncbi.nlm.nih.gov/pubmed/24808911 http://dx.doi.org/10.1186/1678-9199-20-16 |
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