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Age-Related Decrease of Meiotic Cohesins in Human Oocytes

Aneuploidy in fetal chromosomes is one of the causes of pregnancy loss and of congenital birth defects. It is known that the frequency of oocyte aneuploidy increases with the human maternal age. Recent data have highlighted the contribution of cohesin complexes in the correct segregation of meiotic...

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Autores principales: Tsutsumi, Makiko, Fujiwara, Reiko, Nishizawa, Haruki, Ito, Mayuko, Kogo, Hiroshi, Inagaki, Hidehito, Ohye, Tamae, Kato, Takema, Fujii, Takuma, Kurahashi, Hiroki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013030/
https://www.ncbi.nlm.nih.gov/pubmed/24806359
http://dx.doi.org/10.1371/journal.pone.0096710
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author Tsutsumi, Makiko
Fujiwara, Reiko
Nishizawa, Haruki
Ito, Mayuko
Kogo, Hiroshi
Inagaki, Hidehito
Ohye, Tamae
Kato, Takema
Fujii, Takuma
Kurahashi, Hiroki
author_facet Tsutsumi, Makiko
Fujiwara, Reiko
Nishizawa, Haruki
Ito, Mayuko
Kogo, Hiroshi
Inagaki, Hidehito
Ohye, Tamae
Kato, Takema
Fujii, Takuma
Kurahashi, Hiroki
author_sort Tsutsumi, Makiko
collection PubMed
description Aneuploidy in fetal chromosomes is one of the causes of pregnancy loss and of congenital birth defects. It is known that the frequency of oocyte aneuploidy increases with the human maternal age. Recent data have highlighted the contribution of cohesin complexes in the correct segregation of meiotic chromosomes. In mammalian oocytes, cohesion is established during the fetal stages and meiosis-specific cohesin subunits are not replenished after birth, raising the possibility that the long meiotic arrest of oocytes facilitates a deterioration of cohesion that leads to age-related increases in aneuploidy. We here examined the cohesin levels in dictyate oocytes from different age groups of humans and mice by immunofluorescence analyses of ovarian sections. The meiosis-specific cohesin subunits, REC8 and SMC1B, were found to be decreased in women aged 40 and over compared with those aged around 20 years (P<0.01). Age-related decreases in meiotic cohesins were also evident in mice. Interestingly, SMC1A, the mitotic counterpart of SMC1B, was substantially detectable in human oocytes, but little expressed in mice. Further, the amount of mitotic cohesins of mice slightly increased with age. These results suggest that, mitotic and meiotic cohesins may operate in a coordinated way to maintain cohesions over a sustained period in humans and that age-related decreases in meiotic cohesin subunits impair sister chromatid cohesion leading to increased segregation errors.
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spelling pubmed-40130302014-05-09 Age-Related Decrease of Meiotic Cohesins in Human Oocytes Tsutsumi, Makiko Fujiwara, Reiko Nishizawa, Haruki Ito, Mayuko Kogo, Hiroshi Inagaki, Hidehito Ohye, Tamae Kato, Takema Fujii, Takuma Kurahashi, Hiroki PLoS One Research Article Aneuploidy in fetal chromosomes is one of the causes of pregnancy loss and of congenital birth defects. It is known that the frequency of oocyte aneuploidy increases with the human maternal age. Recent data have highlighted the contribution of cohesin complexes in the correct segregation of meiotic chromosomes. In mammalian oocytes, cohesion is established during the fetal stages and meiosis-specific cohesin subunits are not replenished after birth, raising the possibility that the long meiotic arrest of oocytes facilitates a deterioration of cohesion that leads to age-related increases in aneuploidy. We here examined the cohesin levels in dictyate oocytes from different age groups of humans and mice by immunofluorescence analyses of ovarian sections. The meiosis-specific cohesin subunits, REC8 and SMC1B, were found to be decreased in women aged 40 and over compared with those aged around 20 years (P<0.01). Age-related decreases in meiotic cohesins were also evident in mice. Interestingly, SMC1A, the mitotic counterpart of SMC1B, was substantially detectable in human oocytes, but little expressed in mice. Further, the amount of mitotic cohesins of mice slightly increased with age. These results suggest that, mitotic and meiotic cohesins may operate in a coordinated way to maintain cohesions over a sustained period in humans and that age-related decreases in meiotic cohesin subunits impair sister chromatid cohesion leading to increased segregation errors. Public Library of Science 2014-05-07 /pmc/articles/PMC4013030/ /pubmed/24806359 http://dx.doi.org/10.1371/journal.pone.0096710 Text en © 2014 Tsutsumi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tsutsumi, Makiko
Fujiwara, Reiko
Nishizawa, Haruki
Ito, Mayuko
Kogo, Hiroshi
Inagaki, Hidehito
Ohye, Tamae
Kato, Takema
Fujii, Takuma
Kurahashi, Hiroki
Age-Related Decrease of Meiotic Cohesins in Human Oocytes
title Age-Related Decrease of Meiotic Cohesins in Human Oocytes
title_full Age-Related Decrease of Meiotic Cohesins in Human Oocytes
title_fullStr Age-Related Decrease of Meiotic Cohesins in Human Oocytes
title_full_unstemmed Age-Related Decrease of Meiotic Cohesins in Human Oocytes
title_short Age-Related Decrease of Meiotic Cohesins in Human Oocytes
title_sort age-related decrease of meiotic cohesins in human oocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013030/
https://www.ncbi.nlm.nih.gov/pubmed/24806359
http://dx.doi.org/10.1371/journal.pone.0096710
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