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Exploring Codon Optimization and Response Surface Methodology to Express Biologically Active Transmembrane RANKL in E. coli

Receptor activator of nuclear factor (NF)-κB ligand (RANKL), a master cytokine that drives osteoclast differentiation, activation and survival, exists in both transmembrane and extracellular forms. To date, studies on physiological role of RANKL have been mainly carried out with extracellular RANKL...

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Detalles Bibliográficos
Autores principales: Maharjan, Sushila, Singh, Bijay, Bok, Jin-Duck, Kim, Jeong-In, Jiang, Tao, Cho, Chong-Su, Kang, Sang-Kee, Choi, Yun-Jaie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014495/
https://www.ncbi.nlm.nih.gov/pubmed/24809485
http://dx.doi.org/10.1371/journal.pone.0096259