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A Novel TetR-Regulating Peptide Turns off rtTA-Mediated Activation of Gene Expression
Conditional regulation of gene expression is a powerful and indispensable method for analyzing gene function. The “Tet-On” system is a tool widely used for that purpose. Here, the transregulator rtTA mediates expression of a gene of interest after addition of the small molecule effector doxycycline....
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014509/ https://www.ncbi.nlm.nih.gov/pubmed/24810590 http://dx.doi.org/10.1371/journal.pone.0096546 |
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author | Schmidt, Sebastian Berens, Christian Klotzsche, Marcus |
author_facet | Schmidt, Sebastian Berens, Christian Klotzsche, Marcus |
author_sort | Schmidt, Sebastian |
collection | PubMed |
description | Conditional regulation of gene expression is a powerful and indispensable method for analyzing gene function. The “Tet-On” system is a tool widely used for that purpose. Here, the transregulator rtTA mediates expression of a gene of interest after addition of the small molecule effector doxycycline. Although very effective in rapidly turning on gene expression, the system is hampered by the long half-life of doxycycline which makes shutting down gene expression rapidly very difficult to achieve. We isolated an rtTA-binding peptide by in vivo selection that acts as a doxycycline antagonist and leads to rapid and efficient shut down of rtTA-mediated reporter gene expression in a human cell line. This peptide represents the basis for novel effector molecules which complement the “Tet-system” by enabling the investigator to rapidly turn gene expression not just on at will, but now also off. |
format | Online Article Text |
id | pubmed-4014509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40145092014-05-14 A Novel TetR-Regulating Peptide Turns off rtTA-Mediated Activation of Gene Expression Schmidt, Sebastian Berens, Christian Klotzsche, Marcus PLoS One Research Article Conditional regulation of gene expression is a powerful and indispensable method for analyzing gene function. The “Tet-On” system is a tool widely used for that purpose. Here, the transregulator rtTA mediates expression of a gene of interest after addition of the small molecule effector doxycycline. Although very effective in rapidly turning on gene expression, the system is hampered by the long half-life of doxycycline which makes shutting down gene expression rapidly very difficult to achieve. We isolated an rtTA-binding peptide by in vivo selection that acts as a doxycycline antagonist and leads to rapid and efficient shut down of rtTA-mediated reporter gene expression in a human cell line. This peptide represents the basis for novel effector molecules which complement the “Tet-system” by enabling the investigator to rapidly turn gene expression not just on at will, but now also off. Public Library of Science 2014-05-08 /pmc/articles/PMC4014509/ /pubmed/24810590 http://dx.doi.org/10.1371/journal.pone.0096546 Text en © 2014 Schmidt et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Schmidt, Sebastian Berens, Christian Klotzsche, Marcus A Novel TetR-Regulating Peptide Turns off rtTA-Mediated Activation of Gene Expression |
title | A Novel TetR-Regulating Peptide Turns off rtTA-Mediated Activation of Gene Expression |
title_full | A Novel TetR-Regulating Peptide Turns off rtTA-Mediated Activation of Gene Expression |
title_fullStr | A Novel TetR-Regulating Peptide Turns off rtTA-Mediated Activation of Gene Expression |
title_full_unstemmed | A Novel TetR-Regulating Peptide Turns off rtTA-Mediated Activation of Gene Expression |
title_short | A Novel TetR-Regulating Peptide Turns off rtTA-Mediated Activation of Gene Expression |
title_sort | novel tetr-regulating peptide turns off rtta-mediated activation of gene expression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014509/ https://www.ncbi.nlm.nih.gov/pubmed/24810590 http://dx.doi.org/10.1371/journal.pone.0096546 |
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