Cargando…

Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends

Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines w...

Descripción completa

Detalles Bibliográficos
Autores principales: Shimizu, Ernesto, Macías, Analía, Paolicchi, Fernando, Magnano, Gabriel, Zapata, Laura, Fernández, Analía, Canal, Ana, Garbaccio, Sergio, Cataldi, Angel, Caimi, Karina, Zumárraga, Martín
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015263/
https://www.ncbi.nlm.nih.gov/pubmed/24676658
http://dx.doi.org/10.1590/0074-0276140292
_version_ 1782315306912317440
author Shimizu, Ernesto
Macías, Analía
Paolicchi, Fernando
Magnano, Gabriel
Zapata, Laura
Fernández, Analía
Canal, Ana
Garbaccio, Sergio
Cataldi, Angel
Caimi, Karina
Zumárraga, Martín
author_facet Shimizu, Ernesto
Macías, Analía
Paolicchi, Fernando
Magnano, Gabriel
Zapata, Laura
Fernández, Analía
Canal, Ana
Garbaccio, Sergio
Cataldi, Angel
Caimi, Karina
Zumárraga, Martín
author_sort Shimizu, Ernesto
collection PubMed
description Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.
format Online
Article
Text
id pubmed-4015263
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Instituto Oswaldo Cruz, Ministério da Saúde
record_format MEDLINE/PubMed
spelling pubmed-40152632014-05-21 Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends Shimizu, Ernesto Macías, Analía Paolicchi, Fernando Magnano, Gabriel Zapata, Laura Fernández, Analía Canal, Ana Garbaccio, Sergio Cataldi, Angel Caimi, Karina Zumárraga, Martín Mem Inst Oswaldo Cruz Articles Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism. Instituto Oswaldo Cruz, Ministério da Saúde 2014-03-05 2014-04 /pmc/articles/PMC4015263/ /pubmed/24676658 http://dx.doi.org/10.1590/0074-0276140292 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Shimizu, Ernesto
Macías, Analía
Paolicchi, Fernando
Magnano, Gabriel
Zapata, Laura
Fernández, Analía
Canal, Ana
Garbaccio, Sergio
Cataldi, Angel
Caimi, Karina
Zumárraga, Martín
Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
title Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
title_full Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
title_fullStr Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
title_full_unstemmed Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
title_short Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
title_sort genotyping mycobacterium bovis from cattle in the central pampas of argentina: temporal and regional trends
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015263/
https://www.ncbi.nlm.nih.gov/pubmed/24676658
http://dx.doi.org/10.1590/0074-0276140292
work_keys_str_mv AT shimizuernesto genotypingmycobacteriumbovisfromcattleinthecentralpampasofargentinatemporalandregionaltrends
AT maciasanalia genotypingmycobacteriumbovisfromcattleinthecentralpampasofargentinatemporalandregionaltrends
AT paolicchifernando genotypingmycobacteriumbovisfromcattleinthecentralpampasofargentinatemporalandregionaltrends
AT magnanogabriel genotypingmycobacteriumbovisfromcattleinthecentralpampasofargentinatemporalandregionaltrends
AT zapatalaura genotypingmycobacteriumbovisfromcattleinthecentralpampasofargentinatemporalandregionaltrends
AT fernandezanalia genotypingmycobacteriumbovisfromcattleinthecentralpampasofargentinatemporalandregionaltrends
AT canalana genotypingmycobacteriumbovisfromcattleinthecentralpampasofargentinatemporalandregionaltrends
AT garbacciosergio genotypingmycobacteriumbovisfromcattleinthecentralpampasofargentinatemporalandregionaltrends
AT cataldiangel genotypingmycobacteriumbovisfromcattleinthecentralpampasofargentinatemporalandregionaltrends
AT caimikarina genotypingmycobacteriumbovisfromcattleinthecentralpampasofargentinatemporalandregionaltrends
AT zumarragamartin genotypingmycobacteriumbovisfromcattleinthecentralpampasofargentinatemporalandregionaltrends