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Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines w...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015263/ https://www.ncbi.nlm.nih.gov/pubmed/24676658 http://dx.doi.org/10.1590/0074-0276140292 |
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author | Shimizu, Ernesto Macías, Analía Paolicchi, Fernando Magnano, Gabriel Zapata, Laura Fernández, Analía Canal, Ana Garbaccio, Sergio Cataldi, Angel Caimi, Karina Zumárraga, Martín |
author_facet | Shimizu, Ernesto Macías, Analía Paolicchi, Fernando Magnano, Gabriel Zapata, Laura Fernández, Analía Canal, Ana Garbaccio, Sergio Cataldi, Angel Caimi, Karina Zumárraga, Martín |
author_sort | Shimizu, Ernesto |
collection | PubMed |
description | Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism. |
format | Online Article Text |
id | pubmed-4015263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-40152632014-05-21 Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends Shimizu, Ernesto Macías, Analía Paolicchi, Fernando Magnano, Gabriel Zapata, Laura Fernández, Analía Canal, Ana Garbaccio, Sergio Cataldi, Angel Caimi, Karina Zumárraga, Martín Mem Inst Oswaldo Cruz Articles Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism. Instituto Oswaldo Cruz, Ministério da Saúde 2014-03-05 2014-04 /pmc/articles/PMC4015263/ /pubmed/24676658 http://dx.doi.org/10.1590/0074-0276140292 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Shimizu, Ernesto Macías, Analía Paolicchi, Fernando Magnano, Gabriel Zapata, Laura Fernández, Analía Canal, Ana Garbaccio, Sergio Cataldi, Angel Caimi, Karina Zumárraga, Martín Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends |
title | Genotyping Mycobacterium bovis from cattle
in the Central Pampas of Argentina: temporal and regional trends |
title_full | Genotyping Mycobacterium bovis from cattle
in the Central Pampas of Argentina: temporal and regional trends |
title_fullStr | Genotyping Mycobacterium bovis from cattle
in the Central Pampas of Argentina: temporal and regional trends |
title_full_unstemmed | Genotyping Mycobacterium bovis from cattle
in the Central Pampas of Argentina: temporal and regional trends |
title_short | Genotyping Mycobacterium bovis from cattle
in the Central Pampas of Argentina: temporal and regional trends |
title_sort | genotyping mycobacterium bovis from cattle
in the central pampas of argentina: temporal and regional trends |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015263/ https://www.ncbi.nlm.nih.gov/pubmed/24676658 http://dx.doi.org/10.1590/0074-0276140292 |
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