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Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction

BACKGROUND: There are challenges, when extracting bacterial DNA from specimens for molecular diagnostics, since fecal samples also contain DNA from human cells and many different substances derived from food, cell residues and medication that can inhibit downstream PCR. The purpose of the study was...

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Detalles Bibliográficos
Autores principales: Mirsepasi, Hengameh, Persson, Søren, Struve, Carsten, Andersen, Lee O B, Petersen, Andreas M, Krogfelt, Karen A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015497/
https://www.ncbi.nlm.nih.gov/pubmed/24447346
http://dx.doi.org/10.1186/1756-0500-7-50