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New colorimetric screening assays for the directed evolution of fungal laccases to improve the conversion of plant biomass

BACKGROUND: Fungal laccases are multicopper oxidases with huge applicability in different sectors. Here, we describe the development of a set of high-throughput colorimetric assays for screening laccase libraries in directed evolution studies. RESULTS: Firstly, we designed three colorimetric assays...

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Detalles Bibliográficos
Autores principales: Pardo, Isabel, Chanagá, Xiomara, Vicente, Ana Isabel, Alcalde, Miguel, Camarero, Susana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015961/
https://www.ncbi.nlm.nih.gov/pubmed/24159930
http://dx.doi.org/10.1186/1472-6750-13-90