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Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea

BACKGROUND: This study aims to isolate the α-glucosidase inhibitory compounds from mulberry leaves (Morus atropurpurea Roxb., Moraceae) and to develop an analytical method for quantification of the compounds. METHODS: Four flavonoids, rutin (1), isoquercetin (2), kaempferol-3-O-rutinoside (3) and as...

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Autores principales: Hong, Hin-Chu, Li, Song-Lin, Zhang, Xiao-Qi, Ye, Wen-Cai, Zhang, Qing-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016240/
https://www.ncbi.nlm.nih.gov/pubmed/24125526
http://dx.doi.org/10.1186/1749-8546-8-19
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author Hong, Hin-Chu
Li, Song-Lin
Zhang, Xiao-Qi
Ye, Wen-Cai
Zhang, Qing-Wen
author_facet Hong, Hin-Chu
Li, Song-Lin
Zhang, Xiao-Qi
Ye, Wen-Cai
Zhang, Qing-Wen
author_sort Hong, Hin-Chu
collection PubMed
description BACKGROUND: This study aims to isolate the α-glucosidase inhibitory compounds from mulberry leaves (Morus atropurpurea Roxb., Moraceae) and to develop an analytical method for quantification of the compounds. METHODS: Four flavonoids, rutin (1), isoquercetin (2), kaempferol-3-O-rutinoside (3) and astragalin (4), were isolated by column chromatography from mulberry leaf water extracts (MWE). The α-glucosidase inhibitory activities of MWE and the four isolated compounds were evaluated by a microplate-based in vitro assay. The content of the isolated flavonoids in M. atropurpurea leaves purchased from different local herbal stores or collected in different locations was determined by high performance liquid chromatography. RESULTS: The four flavonoids (1–4) showed α-glucosidase inhibitory activities, with rutin (1) and astragalin (4) showing high α-glucosidase inhibitory activities (IC(50) values of 13.19 ± 1.10 and 15.82 ± 1.11 μM, respectively). The total contents of the four flavonoids were different among eight samples examined, ranging from 4.34 mg/g to 0.53 mg/g. CONCLUSIONS: The four flavonoids in M. atropurpurea leaves could inhibit α-glucosidase activity.
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spelling pubmed-40162402014-05-23 Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea Hong, Hin-Chu Li, Song-Lin Zhang, Xiao-Qi Ye, Wen-Cai Zhang, Qing-Wen Chin Med Research BACKGROUND: This study aims to isolate the α-glucosidase inhibitory compounds from mulberry leaves (Morus atropurpurea Roxb., Moraceae) and to develop an analytical method for quantification of the compounds. METHODS: Four flavonoids, rutin (1), isoquercetin (2), kaempferol-3-O-rutinoside (3) and astragalin (4), were isolated by column chromatography from mulberry leaf water extracts (MWE). The α-glucosidase inhibitory activities of MWE and the four isolated compounds were evaluated by a microplate-based in vitro assay. The content of the isolated flavonoids in M. atropurpurea leaves purchased from different local herbal stores or collected in different locations was determined by high performance liquid chromatography. RESULTS: The four flavonoids (1–4) showed α-glucosidase inhibitory activities, with rutin (1) and astragalin (4) showing high α-glucosidase inhibitory activities (IC(50) values of 13.19 ± 1.10 and 15.82 ± 1.11 μM, respectively). The total contents of the four flavonoids were different among eight samples examined, ranging from 4.34 mg/g to 0.53 mg/g. CONCLUSIONS: The four flavonoids in M. atropurpurea leaves could inhibit α-glucosidase activity. BioMed Central 2013-10-14 /pmc/articles/PMC4016240/ /pubmed/24125526 http://dx.doi.org/10.1186/1749-8546-8-19 Text en Copyright © 2013 Hong et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Hong, Hin-Chu
Li, Song-Lin
Zhang, Xiao-Qi
Ye, Wen-Cai
Zhang, Qing-Wen
Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea
title Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea
title_full Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea
title_fullStr Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea
title_full_unstemmed Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea
title_short Flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea
title_sort flavonoids with α-glucosidase inhibitory activities and their contents in the leaves of morus atropurpurea
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016240/
https://www.ncbi.nlm.nih.gov/pubmed/24125526
http://dx.doi.org/10.1186/1749-8546-8-19
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