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Improved soluble expression of the gene encoding amylolytic enzyme Amo45 by fusion with the mobile-loop-region of co-chaperonin GroES in Escherichia coli

The gene encoding the amylolytic enzyme Amo45, originating from a metagenomic project, was retrieved by a consensus primer-based approach for glycoside hydrolase (GH) family 57 enzymes. Family 57 contains mainly uncharacterized proteins similar to archaeal thermoactive amylopullulanases. For charact...

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Detalles Bibliográficos
Autores principales: Wang, Lei, Watzlawick, Hildegard, Fridjonsson, Olafur, Hreggvidsson, Gudmundur, Altenbuchner, Josef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Informa Healthcare 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017763/
https://www.ncbi.nlm.nih.gov/pubmed/24829536
http://dx.doi.org/10.3109/10242422.2013.858712