A novel, non-radioactive eukaryotic in vitro transcription assay for sensitive quantification of RNA polymerase II activity

BACKGROUND: Many studies of the eukaryotic transcription mechanism and its regulation rely on in vitro assays. Conventional RNA polymerase II transcription assays are based on radioactive labelling of the newly synthesized RNA. Due to the inefficient in vitro transcription, the detection of the RNA...

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Detalles Bibliográficos
Autores principales: Voss, Cristina, Schmitt, Brita, Werner-Simon, Susanne, Lutz, Christian, Simon, Werner, Anderl, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021065/
https://www.ncbi.nlm.nih.gov/pubmed/24694320
http://dx.doi.org/10.1186/1471-2199-15-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021065/
https://www.ncbi.nlm.nih.gov/pubmed/24694320
http://dx.doi.org/10.1186/1471-2199-15-7

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