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Nested PCR detection of malaria directly using blood filter paper samples from epidemiological surveys

BACKGROUND: Nested PCR is considered a sensitive and specific method for detecting malaria parasites and is especially useful in epidemiological surveys. However, the preparation of DNA templates for PCR is often time-consuming and costly. METHODS: A simplified PCR method was developed to directly u...

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Detalles Bibliográficos
Autores principales: Li, Peipei, Zhao, Zhenjun, Wang, Ying, Xing, Hua, Parker, Daniel M, Yang, Zhaoqing, Baum, Elizabeth, Li, Wenli, Sattabongkot, Jetsumon, Sirichaisinthop, Jeeraphat, Li, Shuying, Yan, Guiyun, Cui, Liwang, Fan, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022400/
https://www.ncbi.nlm.nih.gov/pubmed/24884761
http://dx.doi.org/10.1186/1475-2875-13-175