Complete steady-state rate equation for DNA ligase and its use for measuring product kinetic parameters of NAD(+)-dependent DNA ligase from Haemophilus influenzae

BACKGROUND: DNA ligase seals the nicks in the phosphodiester backbone between Okazaki fragments during DNA replication. DNA ligase has an unusual Bi Ter Ping Pong kinetic mechanism. Its substrates in eubacteria are NAD(+) and nicked DNA (nDNA). Its products are nicotinamide mononucleotide (NMN), ade...

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Detalles Bibliográficos
Autor principal: Shapiro, Adam B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022432/
https://www.ncbi.nlm.nih.gov/pubmed/24885075
http://dx.doi.org/10.1186/1756-0500-7-287

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4022432/
https://www.ncbi.nlm.nih.gov/pubmed/24885075
http://dx.doi.org/10.1186/1756-0500-7-287

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