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Optimization of RNA Extraction from Rat Pancreatic Tissue

Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination....

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Detalles Bibliográficos
Autores principales: Dastgheib, Sanaz, Irajie, Cambyz, Assaei, Raheleh, Koohpeima, Farhad, Mokarram, Pooneh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027008/
https://www.ncbi.nlm.nih.gov/pubmed/24850986