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Optimization of RNA Extraction from Rat Pancreatic Tissue
Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination....
Autores principales: | Dastgheib, Sanaz, Irajie, Cambyz, Assaei, Raheleh, Koohpeima, Farhad, Mokarram, Pooneh |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shiraz University of Medical Sciences
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027008/ https://www.ncbi.nlm.nih.gov/pubmed/24850986 |
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