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Vicinal: a method for the determination of ncRNA ends using chimeric reads from RNA-seq experiments
Non-coding (nc)RNAs are important structural and regulatory molecules. Accurate determination of the primary sequence and secondary structure of ncRNAs is important for understanding their functions. During cDNA synthesis, RNA 3′ end stem-loops can self-prime reverse transcription, creating RNA–cDNA...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027162/ https://www.ncbi.nlm.nih.gov/pubmed/24623808 http://dx.doi.org/10.1093/nar/gku207 |