Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA

N.ϕGamma is a strand-specific and site-specific DNA nicking enzyme (YCG↓GT or AC↑CGR). Here we describe the isolation of single and double mutants of N.ϕGamma with attenuated activity. The nicking domains (NDs) of E59A and 11 double mutants were fused to the 5mCG-binding domain of MBD2 and generated...

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Detalles Bibliográficos
Autores principales: Gutjahr, Alice, Xu, Shuang-yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027164/
https://www.ncbi.nlm.nih.gov/pubmed/24609382
http://dx.doi.org/10.1093/nar/gku192

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027164/
https://www.ncbi.nlm.nih.gov/pubmed/24609382
http://dx.doi.org/10.1093/nar/gku192

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