Estimating telomere length from whole genome sequence data

Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot...

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Detalles Bibliográficos
Autores principales: Ding, Zhihao, Mangino, Massimo, Aviv, Abraham, Spector, Tim, Durbin, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027178/
https://www.ncbi.nlm.nih.gov/pubmed/24609383
http://dx.doi.org/10.1093/nar/gku181
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author Ding, Zhihao
Mangino, Massimo
Aviv, Abraham
Spector, Tim
Durbin, Richard
author_facet Ding, Zhihao
Mangino, Massimo
Aviv, Abraham
Spector, Tim
Durbin, Richard
author_sort Ding, Zhihao
collection PubMed
description Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot measurements of the mean length of terminal restriction fragments (mTRFs) and display age-dependent attrition comparably well as mTRFs.
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spelling pubmed-40271782014-05-28 Estimating telomere length from whole genome sequence data Ding, Zhihao Mangino, Massimo Aviv, Abraham Spector, Tim Durbin, Richard Nucleic Acids Res Methods Online Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot measurements of the mean length of terminal restriction fragments (mTRFs) and display age-dependent attrition comparably well as mTRFs. Oxford University Press 2014-05-01 2014-03-07 /pmc/articles/PMC4027178/ /pubmed/24609383 http://dx.doi.org/10.1093/nar/gku181 Text en © The Author(s) 2014. Published by Oxford University Press [on behalf of insert name of society]. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methods Online
Ding, Zhihao
Mangino, Massimo
Aviv, Abraham
Spector, Tim
Durbin, Richard
Estimating telomere length from whole genome sequence data
title Estimating telomere length from whole genome sequence data
title_full Estimating telomere length from whole genome sequence data
title_fullStr Estimating telomere length from whole genome sequence data
title_full_unstemmed Estimating telomere length from whole genome sequence data
title_short Estimating telomere length from whole genome sequence data
title_sort estimating telomere length from whole genome sequence data
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027178/
https://www.ncbi.nlm.nih.gov/pubmed/24609383
http://dx.doi.org/10.1093/nar/gku181
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